Lab Data Validation (LDV)

Objectives of the LDV

Centralize Seed Inspection & Testing Records: To establish a single, unified system for managing all data related to seed lot inspections and various quality tests (Purity, Germination, Emergence, etc.).
Ensure Traceability and Data Integrity: To guarantee that all information throughout the seed testing workflow is accurate, consistent, and fully traceable from the initial receipt of a seed lot to its final report.
Enhance Operational Efficiency: Streamline laboratory workflows through efficient data capture, record management, search functionalities, and report generation, thereby optimizing throughput and resource utilization.
Strengthen Quality Control and Certification Adherence: Deliver reliable and verifiable data essential for informed decision-making regarding seed lot acceptance, retesting protocols,

LDV Demo

🌱 Purity Test

🧪 What is a Seed Purity Test?

A Purity Test in seed technology is a key laboratory procedure used to determine the composition of a seed sample. It identifies the percentage of pure seed, inert matter, other crop seeds, and weed seeds present in the lot. This test ensures the genetic and physical quality of seeds before certification and commercial use.

✅ Why is Purity Testing Important?

Ensures farmers receive clean and quality seeds
Helps maintain seed standards for national certification (as per ISTA or Indian Seed Standards)
Prevents crop contamination from unwanted weed or other crop seeds
Vital for traceability and accountability in seed production

🔍 How the Purity Test is Performed?

Sample Size: Usually 1,000 seeds or a weight-based representative sample is taken.
Components Identified:
- - - - Pure Seed – Seeds that conform to the species stated
      - Inert Matter – Soil, stones, broken seeds, seed parts
      - Other Crop Seeds – Seeds from different crop species
      - Weed Seeds – Harmful or invasive species

🔬Germination & Emergence Test

🧪 What is Germination & Emergence Test?

Germination and Emergence Tests are seed quality assessments that determine a seed’s ability to sprout and grow into a healthy plant.

A Germination Test is conducted in controlled lab conditions (like moist paper or germinator) to check how many seeds develop into normal seedlings.
An Emergence Test is done in soil or sand to see how many seeds can push through and emerge under natural field-like conditions.

These tests help ensure the viability, vigor, and performance of seeds before they are sold, distributed, or certified.

✅ Why Are These Tests Important?

Helps determine seed lot quality before sale or distribution
Identifies low-vigor or dead seeds
Provides data for seed certification and labeling
Ensures farmer confidence in seed performance

🔬 How Germination Testing is Done:

Sample Preparation: Usually 4 replicates of 100 seeds each
Testing Conditions:
- 1. - Temperature and moisture are controlled
    - Light/dark conditions as per crop requirement
Duration: Typically 7–14 days depending on the species
Results Classification:
- 1. - Normal Seedlings
    - Abnormal Seedlings
    - Dead Seeds
    - Hard or Dormant Seeds

🧬 ODV (Other Distinguishable Varieties) Test

🔍 What is ODV?

ODV stands for Other Distinguishable Varieties. It refers to seeds of different varieties or types that can be visually or physically distinguished from the intended seed variety during examination.

🧪What is an ODV Test?

An ODV Test is a seed purity assessment that detects the presence of seeds belonging to other identifiable varieties within a seed lot. It is used to ensure genetic purity and maintain varietal identity, especially in certified and hybrid seed production.

🔬 How the ODV Test is Conducted

Sample Size: 1,000 to 3,000 seeds or as per ISTA/Indian standards
Method:
- Visual inspection under magnifier or stereomicroscope
- Identify seeds differing in shape, color, size, or surface texture
Counting: Total seeds vs. ODV seeds

📐 ODV % Calculation Formula

ODV % = (Weight of Other Varietal Seeds / Total Sample Weight) x 100

📊 Vigour Index Formula

A popular formula used in labs is:

Vigour Index=Germination %×Average Seedling Length (cm)

Example:
If Germination % = 90
Avg. Seedling Length = 12 cm
Then Vigour Index = 90 × 12 = 1080

🌱 VIGOUR TESTING

🎯 Purpose of Vigour Testing

The primary goal of vigour testing is to act as a quality control tool, enabling seed labs and producers to:

Rank seed batches based on their potential performance in the field
Ensure longevity and viability during storage and transport

🔬 What Vigour Testing Reveals

Vigour tests provide valuable insights into:

✅ Speed & Consistency of Germination

✅ Field Emergence Capability

✅ Post-Storage Viability

🧪 Tetrazolium Test

🔬 What is the Tetrazolium Test?

The Tetrazolium Test, often called the T-Test, is a quick biochemical method used to evaluate the viability of seeds by detecting living cells through enzymatic activity.

⚙️ How It Works?

Seeds are soaked and then cut open to expose the embryo tissues.
The tissue is immersed in a solution containing Tetrazolium salt (commonly TTC – 2,3,5-triphenyltetrazolium chloride).
Living cells contain enzymes (dehydrogenases) that reduce the colorless TTC into a red or pink formazan dye.
The intensity and location of staining indicate whether the seed is alive and which parts are viable.